课程文献中心 更多>>

关键词： 瞬时受体电位离子通道蛋白M亚家族4型   靶向药物   预后标志物   离子通道   钙离子   肿瘤  

关键词： 沉默信息调节因子2   人子宫内膜癌   凋亡   迁移  

关键词： Bioinformatics   Colon cancer   Epigenetics   Histone modifications   In vitro experiment   PTEN-induced putative kinase 1  

摘要： 目的: 探讨PTEN诱导假定激酶1(PINK1)基因对结肠癌细胞活性、迁移、凋亡的影响以及对结肠癌细胞的表观调控作用。方法: 利用慢病毒包装技术构建PINK1基因过表达和敲降的HCT116和DLD1结肠癌细胞系,应用定量逆转录聚合酶链反应、蛋白质印迹法验证PINK1基因的表达情况;利用CCK-8法和克隆形成实验检测细胞增殖能力;细胞划痕实验和Transwell小室实验检测细胞迁移能力;Hoechst 33258染色观察细胞凋亡情况;蛋白质印迹法检测组蛋白修饰相关蛋白的表达;染色质转座酶可及性(ATAC)测序技术分析染色质开放状态。结果: PINK1 mRNA在结直肠癌组织中表达下调,结直肠癌组织中PINK1蛋白表达也较正常组织减少。在HCT116和DLD1细胞中,过表达PINK1显著抑制细胞增殖(均P<0.01)和克隆形成(均P<0.05),并降低细胞迁移能力(均P<0.01)。相反,敲低PINK1可促进细胞增殖、克隆形成和迁移(均P<0.05)。PINK1过表达导致凋亡细胞数增加(均P<0.05),伴随抗凋亡蛋白髓样细胞白血病蛋白(MCL)-1、B细胞淋巴瘤(BCL)-2和BCL-XL下调,以及促凋亡蛋白BCL-2相关X蛋白(BAX)上调(均P<0.01),而p53蛋白表达差异无统计学意义(均P>0.05)。同时,PINK1过表达可抑制组蛋白H3第9位赖氨酸三甲基化(H3K9me3)和组蛋白H3第27位赖氨酸三甲基化(H3K27me3)表达,增加组蛋白H3第9位赖氨酸乙酰化(H3K9ac)和组蛋白H3第27位赖氨酸乙酰化(H3K27ac)表达,且PINK1过表达细胞中组蛋白甲基化相关蛋白Zeste基因增强子同源物(EZH)22、EZH1、Zeste基因抑制因子12同源物(SUZ12)以及组蛋白脱乙酰酶3(HDAC3)的蛋白表达均减少(均P<0.01)。ATAC测序证实PINK1过表达结肠癌细胞中测序峰在基因转录起始位点区域明显富集。结论: PINK1能够抑制结肠癌细胞的增殖和迁移能力,促进其凋亡,并影响结肠癌细胞的组蛋白修饰及染色质开放状态。.;OBJECTIVES: To investigate the role of PTEN-induced putative kinase 1 (PINK1) in regulating the viability, migration, and apoptosis of colorectal cancer (CRC) cells, and to explore its potential epigenetic mechanisms. METHODS: PINK1 was overex-pressed or knocked down in HCT116 and DLD1 CRC cell lines using lentiviral vectors, with efficiency verified by qRT-PCR and Western blotting. Cell proliferation was assessed using CCK-8 and colony formation. Cell migration was detected using wound healing and Transwell assays. Apoptosis was assessed using Hoechst 33258 staining. Protein levels of apoptosis-related and histone modification-related markers were analyzed by Western blotting. Genome-wide chromatin accessibility was profiled using assay for transposase-accessible chromatin with sequencing (ATAC-seq). RESULTS: PINK1 expression was significantly downregulated at both mRNA and protein levels in CRC tissues com-pared to normal tissues. PINK1 overexpression inhibited cell proliferation, colony formation, and migration in HCT116 and DLD1 cells (all P<0.05), whereas PINK1 knockdown promoted these mal

关键词： 胶质瘤   神经元   突触   肿瘤微环境   肿瘤进展  

关键词： Incidence   Labor force population   Mortality   Neoplasms   Time trend  

摘要： 目的: 分析2021年浙江省肿瘤登记地区劳动力人口恶性肿瘤的发病和死亡情况及2000—2021年趋势,预测2031年负担,为精准防控提供依据。 方法: 收集2000—2021年浙江省肿瘤登记地区15~64岁人群恶性肿瘤数据,分析不同性别、年龄组、城乡地区及主要癌种发病和死亡情况,中标率采用中国2000年标准人口构成标化,世标率采用Segi's世界人口构成标化。采用Joinpoint回归模型估计时间变化趋势并计算平均年度变化百分比(AAPC),使用自回归移动平均模型(ARIMA)预测2031年负担。 结果: 2021年,浙江省肿瘤登记地区劳动力人口新发恶性肿瘤60 152例,占全年龄组新发恶性肿瘤的51.6%;死亡10 285例,占全年龄组的25.4%。中标发病率为283.4/10万,中标死亡率为39.5/10万。城市地区发病率高于农村地区,死亡率低于农村地区。世标发病率男女比为0.64∶1,世标死亡率男女比为1.76∶1。发病率前3位的癌种是甲状腺癌、肺癌、女性乳腺癌,死亡率前3位的癌种是肺癌、肝癌和女性乳腺癌。2000—2021年,浙江省肿瘤登记地区劳动力人口恶性肿瘤发病率总体呈上升趋势(AAPC=3.61%,P<0.001),尤其是2013年起快速上升;死亡率总体呈下降趋势(AAPC=-1.60%,P<0.001)。男性甲状腺癌和前列腺癌上升趋势显著,AAPC分别为15.62%和15.30%;女性甲状腺癌增长最为突出,AAPC为15.59%(均P<0.05)。发病和死亡双增长的癌种,男性为前列腺癌和结直肠癌,女性为宫颈癌和淋巴瘤。ARIMA模型预测结果显示,到2031年,浙江省肿瘤登记地区劳动力人口恶性肿瘤世标发病率将升至438/10万,世标死亡率将降至39/10万。 结论: 2000—2021年浙江省劳动力人口恶性肿瘤呈现发病率上升、死亡率下降的趋势,应继续推进肿瘤早诊早治、疫苗接种、体重管理、健康生活方式等工作。.;Objective: To analyze the incidence and mortality of malignant tumors among the labor force in cancer registration areas of Zhejiang Province in 2021, as well as the trend from 2000 to 2021, and predict the burden in 2031, in order to provide reference for precise prevention and control. Methods: The data of malignant tumors among the population aged 15-64 in Zhejiang cancer registration areas from 2000 to 2021 were collected. The incidence and mortality rates by gender, age group, urban-rural region, and major cancer types were analyzed. The age-standardized incidence (mortality) rates were calculated using the 2000 China standard population composition and Segi's world population composition, respectively. The Joinpoint regression model was used to estimate the temporal trends and to calculate the average annual percentage change (AAPC), and the auto-regressive integrated moving average model (ARIMA) was applied to predict the burden in 2031. Results: In 2021, 60 152 new cases of malignant tumors were reported among the labor force in the cancer registration areas of Zhejiang Province, accounting for 51.6% of all newly diagnosed malignant tumors across a

关键词： Gene mutation   Pathology   PTEN   PTEN   Thyroid tumors  

摘要： A retrospective analysis of clinicopathological features was conducted on 10 cases of thyroid tumors with PTEN mutations from Shanghai Sixth People′s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Fujian Provincial Hospital, and the Affiliated Hospital of Qingdao University from 2020 to 2024. Histomorphological features were quantified using the Thyroid Histomorphological PTEN Hamartoma Syndrome (PHTS) scoring system(THiPS), immunophenotypic profiling and genetic features were examined via immunohistochemical staining and next-generation sequencing respectively. The cohort included 3 males and 7 females, with the age [M(Q₁,Q₃)] of 68.0(53.0, 74.0) years. Those 10 cases included papillary thyroid carcinomas (3 cases), oncocytic carcinoma of the thyroid (2 cases), follicular thyroid carcinoma (1 case), differentiated high-grade thyroid carcinoma (1 case), and thyroid low risk neoplasm (3 cases). The cases in this study had low THiPS scores [(1.0±1.15) scores], and all lacked the characteristic morphological features of PHTS. Immunohistochemical staining showed that loss of PTEN expression was confirmed in 7 cases, positive expression was found in 3 cases with non-sense PTEN mutation. Molecular analysis revealed co-mutations with PTEN in 80% of cases (8/10), including BRAF^V600E(3 cases), TERT promoter (4 cases), and TP53 mutations (2 cases), while 2 cases harbored PTEN-only mutations. Follow-up data were obtained for 6 cases, with a follow-up period of 15.0 (9.0, 20.0) months. Among them, 1 case was initially diagnosed with OCT pulmonary metastasis, while the remaining 5 cases showed no recurrence or metastatic lesions during the follow-up period. The THiPS score has limited diagnostic value for thyroid tumors with PTEN alteration. Negative PTEN immunohistochemical staining may suggest a PTEN mutation, but positive staining does not exclude a genetic alteration. PTEN gene mutations can be combined with BRAF, TE

关键词： Food for special medical purpose   Neoplasms   Nutrition therapy   Supportive care  

摘要： 肿瘤患者发生营养不良和代谢紊乱的比例较高,而营养不良对患者的治疗和预后都有显著影响,造成了沉重的疾病和经济负担。近年来,随着对肿瘤患者营养状况的认知提升,特别是营养不良带来的多种负面影响,凸显了临床治疗不足和对不良反应的认识不足,导致肿瘤患者专用特殊医学用途食品使用的缺乏以及不规范。为进一步推动肿瘤患者专用特殊医学用途食品的临床和家庭规范应用,专家组收集整理近年来的高质量证据,并研讨总结临床应用意见,综合形成《特殊医学用途肿瘤全营养配方食品临床应用专家共识(2025版)》,旨在推动肿瘤营养治疗规范化,满足肿瘤患者的特殊营养需求,提高临床对肿瘤特医食品的应用认知,提供详细实用的临床操作指导。.;Malnutrition and metabolic disorders occur in a high proportion of oncology patients, and malnutrition has a significant impact on both treatment and prognosis, resulting in a heavy disease and economic burden. In recent years, as awareness of the nutritional status of oncology patients has improved, especially the multiple negative effects of malnutrition, it has highlighted the lack of clinical treatment and insufficient knowledge of adverse effects, leading to the lack of special medical use foods for oncology patients as well as irregularities in their use. In order to further promote the clinical and family standardised application of special medical purpose foods for oncology patients, the expert group collects and collates high-quality evidence in recent years, and discusses and summarises the opinions on clinical application, and consolidates the consensus "Expert consensus on clinical application of whole nutritional oncology foods for special medical purposes (2025 edition)", aiming to promote the standardisation of oncology nutritional treatment, meet the special nutritional needs of oncology patients, improve the clinical knowledge on the application of special medical purpose foods for oncology, and provide detailed and practical guidance on clinical operation.

关键词： 逆向肿瘤心脏病学   心血管疾病   心肌梗死   心脏分泌因子  

关键词： Lipid metabolism   p53   SOAT1   Tumor  

摘要： 【中文题目:p53-SOAT1轴:肿瘤相关脂质代谢调控
与治疗新靶点】 【中文摘要:癌症治疗是全球医学面临的重大挑战,其复杂的分子机制和药物耐药性是制约治疗效果的关键因素。异常的脂质代谢是肿瘤的重要特征之一,为肿瘤细胞的生长、增殖、迁移和侵袭提供代谢支持。肿瘤抑制因子p53蛋白和甾醇O-酰基转移酶1(sterol O-acyltransferase 1, SOAT1)在调控细胞脂质代谢中发挥重要作用,与多种肿瘤的发生发展及预后密切相关。p53蛋白通过多条信号通路调控肿瘤脂质代谢,而SOAT1作为胆固醇酯化的关键酶,在多种肿瘤中高水平表达并加速肿瘤进展进程。有研究表明p53蛋白与SOAT1可能存在功能性关联作用,协同调控肿瘤细胞的脂质稳态。本文综述了p53蛋白与SOAT1在肿瘤脂质代谢中的研究进展,重点阐述p53-SOAT1轴在肿瘤发生发展中的潜在作用机制,并展望其作为癌症治疗靶点的应用前景。
】 【中文关键词:p53;脂质代谢;SOAT1;肿瘤】.;Cancer treatment is a significant challenge facing global medicine, with complex molecular mechanisms and drug resistance being key factors limiting treatment outcomes. Abnormal lipid metabolism is one of the important characteristics of tumors, providing metabolic support for the growth, proliferation, migration, and invasion of tumor cells. Tumor suppressor p53 protein and sterol O-acyltransferase 1 (SOAT1) play an important role in regulating cellular lipid metabolism and are closely related to the occurrence, development, and prognosis of various tumors. p53 protein regulates tumor lipid metabolism through multiple signaling pathways, while SOAT1, as a key enzyme in cholesterol esterification, is highly expressed in many tumors and accelerates tumor progression. Recent studies have shown that there may be a functional association between p53 protein and SOAT1, coordinating the regulation of lipid homeostasis in tumor cells. This article reviews the research progress on p53 protein and SOAT1 in tumor lipid metabolism, focusing on the potential mechanisms of action of the p53-SOAT1 axis in tumor development, and prospects its application prospects as a target for cancer treatment.
.