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关键词： 施工整合   肿瘤中心   医疗专项   流程优化   智慧医院  

关键词： Gene expression regulation, neoplastic   LASS2 gene   Neoplasm invasiveness   Prostatic neoplasms   Vacuolar proton-translocating ATPases  

摘要： Objective To explore the effects and the molecular mechanisms of homo sapiens longevity assurance homolog 2 of yeast LAG1(LASS2) dephosphorylation on the biological functions of prostate cancer cells. Methods Firstly, we examined the expression profiles of LASS2 by immunohistochemical staining using microarray sections from 90 human patients with prostate cancer;then FLAG-tagged LASS2 plasmid was transferred into HEK 293T cells and phosphorylation sites was detected by mass spectrometry. Furthermore, we constructed five phosphorylation-deficient mutants of LASS2 and stably transfected the variants to human prostate cancer cell line PC-3M-1E8 cell with high metastatic potential. The cell biology functions of LASS2 and its five mutants were studied using growth curve, MTT assay, plate colony formation assay, wound migration assay, matrigel invasion study and flow cytometry;and the effect of LASS2 and its phosphorylation-deficient mutants on the physical interaction between LASS2 and ATP6V0C (C subunit of V0 domain of the vacuolar ATPase), ATP6V0C expression, vacuolar ATPase (V-ATPase) activity, extracellular hydrogen ion concentration and secretion of active matrix metalloproteinase 2(MMP-2) was detected. Finally, we examined the effect of protein phosphatase inhibitor calyculin A on growth, migration and invasion of aggressive prostate cancer cells. Results LASS2 levels decreased with increasing Gleason scores of prostate cancer tissues by immunohistochemical staining;moreover, proteome analysis by mass spectrometry had identified that three residues in the C-terminal region of LASS2(Ser-341, Ser-348, and Ser-349) were phosphorylated. Dephosphorylation of LASS2 at serine residue 348 significantly enhanced growth, migration (from 49.11%±5.62% to 74.28%±8.77%, P < 0.001) and invasion (from 129.67±13.65 to 206.67±13.50, P < 0.001) of prostate cancer cells, decreased S phase arrest (from 44.17% to 37.90%, P < 0.05) and inhibited cell apoptosis (from 48.540%±0.269% to 29.

关键词： Adoptive cell therapy   Antibody peptide conjugate   Breast neoplasms   Cold tumor mouse model   Immunotherapy   Programmed cell death protein 1   Stomach neoplasms  

摘要： 目的: 探讨程序性细胞死亡受体1(PD-1)单抗内化精氨酸-甘氨酸-天冬氨酸多肽(iRGD肽)偶联物[αPD-1-(iRGD)2]联合过继性T细胞治疗(ACT)对“冷肿瘤”免疫微环境的调控作用及其协同抗肿瘤效应。 方法: 采用糖基化定点偶联技术构建αPD-1-(iRGD)2。在HGC27胃癌细胞系常规细胞培养及三维培养模型中,将该肿瘤细胞分为对照组、PD-1单抗单药组(αPD-1组)、PD-1单抗及游离iRGD肽组(αPD-1+iRGD组)以及偶联药物组[αPD-1-(iRGD)2组],评估其对T细胞穿透能力及肿瘤杀伤效力的影响,流式细胞术检测T细胞活化标志物(CD25、CD69)及效应分子的表达特征。通过构建经典“冷肿瘤”模型——BALB/c小鼠4T1乳腺癌皮下瘤模型,分组同前,评估αPD-1-(iRGD)2 联合ACT的抑瘤效果,并运用流式细胞术分析小鼠肿瘤、淋巴结、脾脏组织中T细胞、巨噬细胞等肿瘤微环境变化情况。通过小动物活体T细胞体内实时近红外荧光成像系统动态监测荧光标记的过继T细胞体内分布情况,流式细胞术分析肿瘤浸润淋巴细胞(TIL)表型变化及耗竭标志物(TIM3)表达水平。 结果: αPD-1-(iRGD)2显著增强T细胞穿透HGC27胃癌细胞肿瘤球体的能力,30 min时中心荧光强度高于αPD-1组(P=0.008),在HGC27胃癌细胞常规培养条件及三维培养体系中,T细胞的杀伤比例均高于对照组(均P<0.05)。αPD-1-(iRGD)2介导T细胞激活的竞争性抑制实验流式细胞术分析结果显示,αPD-1-(iRGD)2组颗粒酶B(GZMB)⁺T细胞、人干扰素γ(IFN-γ)⁺T细胞及CD25⁺CD69⁺T细胞比例均高于对照组(均P<0.05)。在4T1乳腺癌皮下瘤小鼠模型中,αPD-1-(iRGD)2联合ACT治疗后肿瘤体积小于对照组(P<0.001),肿瘤内CD8⁺T细胞数量高于αPD-1组(P=0.001),GZMB⁺和IFN-γ⁺CD8⁺T细胞比例分别升高至61.98%±1.80%和58.70%±2.15%(均P<0.05)。近红外体内成像证实,αPD-1-(iRGD)2组的瘤区荧光信号均高于αPD-1组和αPD-1+iRGD组(均P<0.05)。肿瘤组织流式细胞表型分析发现,与αPD-1组相比,αPD-1-(iRGD)2组TIM3⁺耗竭T细胞比例降低,CD44⁺CD62L⁺记忆表型比例升高(均P<0.05)。 结论: PD-1单抗iRGD偶联物通过增强过继T细胞的瘤内浸润能力,改善免疫抑制微环境及维持T细胞效应记忆表型,显著提升“冷肿瘤”对ACT的治疗响应。.;Objective: To explore how the programmed cell death protein 1(PD-1) antibody-internalizing Arg-Gly-Asp peptide conjugate [αPD-1-(iRGD)2], combined with adoptive T cell therapy (ACT), modulates the cold tumor microenvironment and exerts synergistic anti-tumor effects. Methods: αPD-1-(iRGD)2 was synthesized with glycosylation engineering. The penetrability and tumor-cytotoxicity enhancement of αPD-1-(iRGD)2 towards T cells were analyzed through flow cytometry in both conventional and 3D cell culture conditions for HGC27 cells, divided into the control group, the PD-1 monoclonal antibody monotherapy group (αPD-1 group), the PD-1 monoclonal antibody combined with free iRGD peptide group (αPD-1 iRGD group), and the conjugated drug group [αPD-1-(iRGD)2 group]. The biomarkers of T cell activation (CD25 and CD69) and effector function (GZMB、IFN-γ) were also characterized by flow cytom

摘要： 随着人口老龄化的加剧,老年肿瘤患者的数量日益增多,其静脉治疗需求也随之增长。PICC 置管作为老年肿瘤患者重要的静脉治疗途径,虽能有效减少频繁穿刺带来的痛苦,但其相关并发症如血流感染、血栓形成等仍不容忽视。老年患者因生理机能下降,对并发症的耐受性降低,护理难度增加。因此,提升PICC 置管后静脉治疗的耐受性,成为保障老年肿瘤患者治疗效果与生活质量的关键。本文旨在探讨通过个性化护理方案、系统教育培训及心理支持等综合护理干预措施,改善老年肿瘤患者PICC 置管后的静脉治疗耐受性,为临床护理实践提供参考。

摘要： 肿瘤是一类由多种因素引起的细胞异常增生性疾病,严重威胁人类健康。传统的肿瘤治疗方法主要包括手术切除、放疗和化疗,但这些方法存在许多局限性,如手术创伤大、放疗对正常组织的损伤、化疗药物的毒性等。随着分子生物学的快速发展,人们对肿瘤发病机制的认识逐渐深入,靶向治疗作为一种新型的治疗手段应运而生。靶向治疗是指通过特定的分子靶点,使用针对性药物阻断肿瘤的生长和扩散,从而达到治疗肿瘤的目的。这种治疗方法具有高效、低毒的特点,为肿瘤治疗提供了新的希望。

关键词： 共情疲劳   职业认同   心理韧性   肿瘤医院护士   教育路径  

摘要： 随着医疗体系改革的深化及肿瘤发病率的上升,肿瘤医院护士面临的共情疲劳问题日益突出,严重影响其心理健康与护理质量。本研究采用混合研究方法,从职业认同、心理韧性与教育培训三个维度,系统探讨缓解共情疲劳的有效策略。研究显示,通过系统化的教育培训,包括职业认同培养、心理韧性训练及持续职业教育,可显著增强护士的职业认同感与心理韧性,进而有效缓解共情疲劳。本研究为医疗机构开展心理健康支持与人力资源管理提供了理论与实践参考。

关键词： 胃肠道肿瘤   HDAC3   DNA损伤应答   HDAC抑制剂  

摘要： 胃肠道肿瘤是常见且致死率较高的恶性肿瘤，其发病与多种因素有关，主要包括食管癌、胃癌、肝癌、结直肠癌和胰腺癌等类型。组蛋白去乙酰化酶3（histone deacetylase 3，HDAC3）是Ⅰ类HDAC的重要成员，作为表观遗传调控枢纽在DNA损伤应答（DNA damage response，DDR）中发挥着重要功能，其在肿瘤发生发展中的调控作用日益受到关注。论文综述了HDAC3在胃肠道肿瘤发生发展中的作用及其抑制剂在化疗中的研究进展，旨在为临床精准治疗提供依据。

关键词： 肠道菌群   真菌   消化道肿瘤  

摘要： 肠道菌群是人体微生态系统重要组成部分。现有研究表明肠道共生菌群对人体屏障功能、维生素合成、代谢、神经活动、药物代谢以及炎症和免疫等方面具有重要作用，而肠道菌群紊乱失调与恶性肿瘤、炎症性肠病等诸多疾病息息相关。相比肠道细菌群，肠道真菌群占较小比例，但其对人体健康与疾病的影响不容忽视。该综述阐述了肠道真菌群的组成和作用，总结了其与消化道肿瘤发生发展的关系，为后续研究提供参考。

摘要： 消化道肿瘤手术是治疗消化道恶性肿瘤的主要方式,但术后常伴随康复缓慢及并发症。术后早期营养护理已被证实能显著改善康复效果。近年来,个性化的早期营养干预被认为能加快术后恢复,改善营养状态,降低并发症发生率。本研究旨在探讨术后早期营养护理对消化道肿瘤患者的康复影响,分析其对恢复、营养状态、并发症及生化指标的作用,为临床提供有效的护理干预策略,促进术后护理的发展。

摘要： Multiparameter flow cytometry (MFC) has proven its value as a complementary diagnostic tool for myelodysplastic neoplasms (MDS). It is almost universally adopted in everyday clinical practice for assessing patients with cytopenia and suspected MDS through simplified scoring systems or elaborate analytical strategies for detecting immunophenotypical dysplastic features in every hematopoietic cell lineage in the bone marrow (BM). Current guidelines recommend flow cytometric analysis as part of the diagnostic assessment of patients with cytopenia suspected of MDS. We reviewed the existing knowledge and recent advances in the field concerning ①the optimal methods of sample processing and handling, ②antibody panels and fluorochromes, ③the analysis of several BM cell subsets, such as myeloid precursors, maturing granulocytic and monocytic components, and erythropoiesis, and ④MFC scoring systems for immunophenotypic signs of myelodysplasia. 2025, Chinese Medical Association